电离辐射通过转化生长因子-β-介导的上皮-间质转换来促进癌细胞的侵袭迁到

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

摘要 ：聚焦电离辐射前提可通过转化生长因子-β（TGF-β）-抑制作用的结缔组织-间质转换 (EMT)来有利于免疫系统的来袭迁离。使用总额2Gy(60)Coγ新线光照源自人类器官的6种免疫系统，记录与EMT就其的变异，这包括分别并用显微镜核心技术，抗原质区别于新方法则，免疫荧光核心技术，划痕测试和Transwell小室测试来注意到并检测细胞膜组织形态，EMT记号，来袭迁离控制能力等。运用于酶联免疫吸附法则检测这些免疫系统中都TGF-β抗原水平，并用比如说抑制作用剂SB431542来分析报告TGF-β信号通路在电离辐射EMT中都的作用。经过总额为2Gy光照的免疫系统中都存在间叶细胞膜的表达，与所谓光照组相比其结缔组织记号减少，间叶细胞膜记号增加，同时其来袭移出控制能力提升，TGF-β抗原水平也提高。进一步断定由A549电离辐射诱导的EMT可通过对TGF-β信号抑制作用发生逆转。这些结果表明TGF-β抑制作用的EMT在电离辐射诱导提升免疫系统来袭移出控制能力中都起着关键作用。

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